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验证数据展示

  • IF Staining of HepG2 using CL488-16439

    IF Staining of HepG2 using CL488-16439

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ORM1 antibody (CL488-16439) at dilution of 1:200, CL594-Phalloidin (red).

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  • KD/KO验证

CoraLite® Plus 488-conjugated ORM1 Polyclonal antibody

ORM1 Polyclonal Antibody for IF/ICC
Cat No. CL488-16439

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

AGP1, Alpha 1 acid glycoprotein 1, Alpha-1-acid glycoprotein 1, OMD 1, ORM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16439-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16439 targets ORM1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 ORM1 fusion protein Ag9758 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 orosomucoid 1
别名 AGP1, Alpha 1 acid glycoprotein 1, Alpha-1-acid glycoprotein 1, OMD 1, ORM
计算分子量 201 aa, 24 kDa
观测分子量 40-47 kDa
GenBank蛋白编号BC026238
基因名称 ORM1
Gene ID (NCBI) 5004
RRIDAB_3672653
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02763
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ORM1 antibody CL488-16439Download protocol
Standard Protocols
Click here to view our Standard Protocols