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验证数据展示

  • WB analysis using 16439-1-AP (same clone as 16439-1-PBS)

    WB analysis using 16439-1-AP (same clone as 16439-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16439-1-AP (ORM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16439-1-AP (same clone as 16439-1-PBS)

    IHC staining of human liver using 16439-1-AP (same clone as 16439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16439-1-AP (same clone as 16439-1-PBS)

    IHC staining of human liver using 16439-1-AP (same clone as 16439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 16439-1-AP (same clone as 16439-1-PBS)

    IP experiment of human plasma using 16439-1-AP (same clone as 16439-1-PBS)

    IP result of anti-ORM1 (IP:16439-1-AP, 3ug; Detection:16439-1-AP 1:300) with human plasma lysate 100ug. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 16439-1-AP (same clone as 16439-1-PBS)

    FC experiment of HepG2 using 16439-1-AP (same clone as 16439-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ORM1 Polyclonal antibody (16439-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 16439-1-AP (same clone as 16439-1-PBS)

    IHC staining of human colon using 16439-1-AP (same clone as 16439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16439-1-AP (same clone as 16439-1-PBS)

    IF Staining of HepG2 using 16439-1-AP (same clone as 16439-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ORM1 antibody (16439-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ORM1 Polyclonal antibody, PBS Only

ORM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 16439-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16439-1-PBS targets ORM1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9758

Product name: Recombinant human ORM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-201 aa of BC026238

Sequence: NLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 orosomucoid 1
别名
计算分子量 201 aa, 24 kDa
观测分子量 40-47 kDa
GenBank蛋白编号BC026238
基因名称 ORM1
Gene ID (NCBI) 5004
RRIDAB_2878259
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02763
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein.

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