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ORC2L Polyclonal antibody

ORC2L Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12739-1-AP

Print datasheet

Synonyms

ORC2, ORC2L

验证数据展示

  • WB analysis of HepG2 using 12739-1-AP

    WB analysis of HepG2 using 12739-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12739-1-AP (ORC2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12739-1-AP

    IP experiment of HepG2 using 12739-1-AP

    IP result of anti-ORC2L (IP:12739-1-AP, 4ug; Detection:12739-1-AP 1:300) with HepG2 cells lysate 1200ug.

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12739-1-AP targets ORC2L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen ORC2L fusion protein Ag3635 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name origin recognition complex, subunit 2-like (yeast)
Synonyms ORC2, ORC2L
Calculated Molecular Weight 577 aa, 66 kDa
Observed Molecular Weight 70-74 kDa
GenBank Accession NumberBC014834
Gene Symbol ORC2L
Gene ID (NCBI) 4999
RRIDAB_10640799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13416
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ORC2L antibody 12739-1-APDownload protocol
IP protocol for ORC2L antibody 12739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols