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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis of HepG2 using 12739-1-AP

    WB analysis of HepG2 using 12739-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12739-1-AP (ORC2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12739-1-AP

    IP experiment of HepG2 using 12739-1-AP

    IP result of anti-ORC2L (IP:12739-1-AP, 4ug; Detection:12739-1-AP 1:300) with HepG2 cells lysate 1200ug.

ORC2L Polyclonal antibody

ORC2L Polyclonal Antibody for WB, IP, ELISA
Cat No. 12739-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

ORC2, ORC2L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12739-1-AP targets ORC2L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3635

Product name: Recombinant human ORC2L protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 208-578 aa of BC014834

Sequence: SQKIQAQNRVVSAPVGKETPSKRMKRDKTSDLVEEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHKWMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVLDHMGTFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYLIASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHVLRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIPVDNGTLTDFLEKEEEEA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 origin recognition complex, subunit 2-like (yeast)
别名 ORC2, ORC2L
计算分子量 577 aa, 66 kDa
观测分子量 70-74 kDa
GenBank蛋白编号BC014834
基因名称 ORC2L
Gene ID (NCBI) 4999
RRIDAB_10640799
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13416
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ORC2L antibody 12739-1-APDownload protocol
IP protocol for ORC2L antibody 12739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols