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验证数据展示

  • WB analysis of HepG2 using 12739-1-AP

    WB analysis of HepG2 using 12739-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12739-1-AP (ORC2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12739-1-AP

    IP experiment of HepG2 using 12739-1-AP

    IP result of anti-ORC2L (IP:12739-1-AP, 4ug; Detection:12739-1-AP 1:300) with HepG2 cells lysate 1200ug.

ORC2L Polyclonal antibody

ORC2L Polyclonal Antibody for WB, IP, ELISA
Cat No. 12739-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

ORC2, ORC2L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12739-1-AP targets ORC2L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ORC2L fusion protein Ag3635 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 origin recognition complex, subunit 2-like (yeast)
别名 ORC2, ORC2L
计算分子量 577 aa, 66 kDa
观测分子量 70-74 kDa
GenBank蛋白编号BC014834
基因名称 ORC2L
Gene ID (NCBI) 4999
RRIDAB_10640799
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13416
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ORC2L antibody 12739-1-APDownload protocol
IP protocol for ORC2L antibody 12739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols