验证数据展示
经过测试的应用
| Positive WB detected in | human brain |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-60293 targets OPTN/Optineurin in WB, IHC applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag1272 Product name: Recombinant human OPTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-577 aa of BC013876 Sequence: VSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | optineurin |
| 别名 | Optineurin, OPTN, FIP2, FIP 2, FAA4 |
| 计算分子量 | 66 kDa |
| 观测分子量 | 70-74 kDa |
| GenBank蛋白编号 | BC013876 |
| 基因名称 | OPTN |
| Gene ID (NCBI) | 10133 |
| RRID | AB_2883065 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96CV9 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. This antibody is conjugated with Biotin.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin OPTN/Optineurin antibody Biotin-60293 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
