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验证数据展示

  • WB analysis of HeLa using 12970-1-AP (same clone as 12970-1-PBS)

    WB analysis of HeLa using 12970-1-AP (same clone as 12970-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12970-1-AP (OPRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 12970-1-AP (same clone as 12970-1-PBS)

    IHC staining of human gliomas using 12970-1-AP (same clone as 12970-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 12970-1-AP (same clone as 12970-1-PBS)

    IHC staining of human gliomas using 12970-1-AP (same clone as 12970-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12970-1-AP (same clone as 12970-1-PBS)

    IHC staining of mouse brain using 12970-1-AP (same clone as 12970-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12970-1-AP (OPRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 12970-1-AP (same clone as 12970-1-PBS)

    IP experiment of SH-SY5Y using 12970-1-AP (same clone as 12970-1-PBS)

    IP result of anti-OPRL1 (IP:12970-1-AP, 4ug; Detection:12970-1-AP 1:500) with SH-SY5Y cells lysate 2000ug. This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

OPRL1 Polyclonal antibody, PBS Only

OPRL1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12970-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12970-1-PBS targets OPRL1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4079

Product name: Recombinant human OPRL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 71-370 aa of BC038433

Sequence: LVMYVILRHTKMKTATNIYIFNLALADTLVLLTLPFQGTDILLGFWPFGNALCKTVIAIDYYNMFTSTFTLTAMSVDRYVAICHPIRALDVRTSSKAQAVNVAIWALASVVGVPVAIMGSAQVEDEEIECLVEIPTPQDYWGPVFAICIFLFSFIVPVLVISVCYSLMIRRLRGVRLLSGSREKDRNLRRITRLVLVVVAVFVGCWTPVQVFVLAQGLGVQPSSETAVAILRFCTALGYVNSCLNPILYAFLDENFKACFRKFCCASALRRDVQVSDRVRSIAKDVALACKTSETVPRPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 opiate receptor-like 1
别名
计算分子量 370 aa, 41 kDa
观测分子量 45 kDa
GenBank蛋白编号BC038433
基因名称 OPRL1
Gene ID (NCBI) 4987
RRIDAB_10638603
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41146
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The OPRL1 (opioid related nociceptin receptor 1) is the G-protein coupled receptor activated by the endogenou NC (neuropeptide nociceptin) (PMID:7566152). OPRL1 is widely expressed in the central nervous system, including the hippocampal dentate gyrus, cortical areas, striatum, thalamus, and hypothalamus (PMID:19501074). OPRL1 has been found to play an essential role in cognition, and to modulate inflammation and immune responses (PMID:30152845).

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