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  • Featured Product
  • KD/KO Validated

OPRL1 Polyclonal antibody

OPRL1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12970-1-AP

Print datasheet

Synonyms

Kappa type 3 opioid receptor, KOR 3, Nociceptin receptor, NOCIR, OOR, opiate receptor like 1, OPRL1, ORL1, Orphanin FQ receptor

验证数据展示

  • WB analysis of HeLa using 12970-1-AP

    WB analysis of HeLa using 12970-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12970-1-AP (OPRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12970-1-AP

    IHC staining of human gliomas using 12970-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human gliomas using 12970-1-AP

    IHC staining of human gliomas using 12970-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of mouse brain using 12970-1-AP

    IHC staining of mouse brain using 12970-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12970-1-AP (OPRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of SH-SY5Y using 12970-1-AP

    IP experiment of SH-SY5Y using 12970-1-AP

    IP result of anti-OPRL1 (IP:12970-1-AP, 4ug; Detection:12970-1-AP 1:500) with SH-SY5Y cells lysate 2000ug.

  • FC experiment of SH-SY5Y using 12970-1-AP

    FC experiment of SH-SY5Y using 12970-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug OPRL1 antibody (12970-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12970-1-AP targets OPRL1 in WB, IP, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen OPRL1 fusion protein Ag4079 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name opiate receptor-like 1
Synonyms Kappa type 3 opioid receptor, KOR 3, Nociceptin receptor, NOCIR, OOR, opiate receptor like 1, OPRL1, ORL1, Orphanin FQ receptor
Calculated Molecular Weight 370 aa, 41 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC038433
Gene Symbol OPRL1
Gene ID (NCBI) 4987
RRIDAB_10638603
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41146
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The OPRL1 (opioid related nociceptin receptor 1) is the G-protein coupled receptor activated by the endogenou NC (neuropeptide nociceptin) (PMID:7566152). OPRL1 is widely expressed in the central nervous system, including the hippocampal dentate gyrus, cortical areas, striatum, thalamus, and hypothalamus (PMID:19501074). OPRL1 has been found to play an essential role in cognition, and to modulate inflammation and immune responses (PMID:30152845).

实验方案

Product Specific Protocols
WB protocol for OPRL1 antibody 12970-1-APDownload protocol
IHC protocol for OPRL1 antibody 12970-1-APDownload protocol
IP protocol for OPRL1 antibody 12970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IHC

Int Immunopharmacol

PMID: 31865244

Authors - Neili Xu
View Article
humanWB

Cell Death Discov

The nociceptin receptor promotes autophagy through NF-kB signaling and is transcriptionally regulated by E2F1 in HCC.

Authors - Xiaoshuang Zhou
  • KD Validated
View Article
ratWB

Biomed Res Int

PMID: 25276817

Authors - Katarzyna Popiolek-Barczyk
View Article
rat

Biochim Biophys Acta

Pharmacological blockade of CXCR3 by (±)-NBI-74330 reduces neuropathic pain and enhances opioid effectiveness - Evidence from in vivo and in vitro studies.

Authors - Anna Piotrowska
View Article