验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24478-1-AP targets OPN4 in IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | OPN4 fusion protein Ag21643 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | opsin 4 |
| 别名 | Opsin-4, opsin 4, MOP, Melanopsin |
| 计算分子量 | 478 aa, 53 kDa |
| GenBank蛋白编号 | BC113558 |
| 基因名称 | OPN4 |
| Gene ID (NCBI) | 94233 |
| RRID | AB_3085736 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHM6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OPN4, an opsin that was first isolated from the skin of Xenopus laevis in 1998, belongs not to the vertebrate-type opsin family but rather to the invertebrate-type opsins, which are part of the seven-transmembrane G protein-coupled receptor (GPCR) family (PMID:31989708). In humans, OPN4 is expressed in the retinal ganglion cells in addition to part of the brain (PMID:10632589). Moreover, Opn4 functions as a photoreceptor by detecting brightness levels and discriminating visual signals (PMID:22633808). Opn4 also facilitates visual circuits to acquire optimal settings and light adaptation by measuring irradiance levels (PMID:25517373).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for OPN4 antibody 24478-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |