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验证数据展示

  • WB analysis of PC-3 using 12596-1-AP (same clone as 12596-1-PBS)

    WB analysis of PC-3 using 12596-1-AP (same clone as 12596-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12596-1-AP (same clone as 12596-1-PBS)

    IF Staining of HepG2 using 12596-1-AP (same clone as 12596-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OLFM3 antibody (12596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 12596-1-AP (same clone as 12596-1-PBS)

    IHC staining of human liver cancer using 12596-1-AP (same clone as 12596-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 12596-1-AP (same clone as 12596-1-PBS)

    IHC staining of human liver cancer using 12596-1-AP (same clone as 12596-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12596-1-AP (same clone as 12596-1-PBS)

    IHC staining of mouse brain using 12596-1-AP (same clone as 12596-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12596-1-AP (OLFM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

OLFM3 Polyclonal antibody, PBS Only

OLFM3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12596-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Olfactomedin 3, NOE3, Noelin 3, NOELIN3, Noelin-3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12596-1-PBS targets OLFM3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3207

Product name: Recombinant human OLFM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 159-458 aa of BC022531

Sequence: NLSAVLTGIQEEIGAYDYEELHQRVLSLETRLRDCMKKLTCGKLMKITGPVTVKTSGTRFGAWMTDPLASEKNNRVWYMDSYTNNKIVREYKSIADFVSGAESRTYNLPFKWAGTNHVVYNGSLYFNKYQSNIIIKYSFDMGRVLAQRSLEYAGFHNVYPYTWGGFSDIDLMADEIGLWAVYATNQNAGNIVISQLNQDTLEVMKSWSTGYPKRSAGESFMICGTLYVTNSHLTGAKVYYSYSTKTSTYEYTDIPFHNQYFHISMLDYNARDRALCAWNNGHQVLFNVTLFHIIKTEDDT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 olfactomedin 3
别名 Olfactomedin 3, NOE3, Noelin 3, NOELIN3, Noelin-3
计算分子量 458 aa, 53 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC022531
基因名称 OLFM3
Gene ID (NCBI) 118427
RRIDAB_2157228
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PB7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OLFM3, also known as Noelin-3, belongs to a larger family of proteins containing the olfactomedin domain. OLFM3, which is most closely related to OLFM2, is expressed in the retinal ganglion and inner nuclear layers of the adult rat retina (PMID: 12019210).

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