验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, A2780 cells, human liver tissue, mouse liver tissue, NIH/3T3 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
12596-1-AP targets OLFM3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3207 Product name: Recombinant human OLFM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 159-458 aa of BC022531 Sequence: NLSAVLTGIQEEIGAYDYEELHQRVLSLETRLRDCMKKLTCGKLMKITGPVTVKTSGTRFGAWMTDPLASEKNNRVWYMDSYTNNKIVREYKSIADFVSGAESRTYNLPFKWAGTNHVVYNGSLYFNKYQSNIIIKYSFDMGRVLAQRSLEYAGFHNVYPYTWGGFSDIDLMADEIGLWAVYATNQNAGNIVISQLNQDTLEVMKSWSTGYPKRSAGESFMICGTLYVTNSHLTGAKVYYSYSTKTSTYEYTDIPFHNQYFHISMLDYNARDRALCAWNNGHQVLFNVTLFHIIKTEDDT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | olfactomedin 3 |
| 别名 | Olfactomedin 3, NOE3, Noelin 3, NOELIN3, Noelin-3 |
| 计算分子量 | 458 aa, 53 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC022531 |
| 基因名称 | OLFM3 |
| Gene ID (NCBI) | 118427 |
| RRID | AB_2157228 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96PB7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OLFM3, also known as Noelin-3, belongs to a larger family of proteins containing the olfactomedin domain. OLFM3, which is most closely related to OLFM2, is expressed in the retinal ganglion and inner nuclear layers of the adult rat retina (PMID: 12019210).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OLFM3 antibody 12596-1-AP | Download protocol |
| IHC protocol for OLFM3 antibody 12596-1-AP | Download protocol |
| IF protocol for OLFM3 antibody 12596-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
