hIST1 Polyclonal antibody

hIST1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19842-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (3)

IST1, KIAA0174, Charged multivesicular body protein 8, CHMP8, IST1 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, mouse brain tissue, PC-3 cells
Positive IHC detected inmouse stomach tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IST1 Ready-To-Use IHC Kit. IST1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19842-1-AP targets hIST1 in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, CoIP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 hIST1 fusion protein Ag13874 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0174
别名 IST1, KIAA0174, Charged multivesicular body protein 8, CHMP8, IST1 homolog
计算分子量 364 aa, 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC000116
基因名称 hIST1
Gene ID (NCBI) 9798
RRIDAB_2878612
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53990
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for hIST1 antibody 19842-1-APDownload protocol
IHC protocol for hIST1 antibody 19842-1-APDownload protocol
IF protocol for hIST1 antibody 19842-1-APDownload protocol
IP protocol for hIST1 antibody 19842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
IF

Science

Triggered recruitment of ESCRT machinery promotes endolysosomal repair.

Authors - Michael L Skowyra
humanIF

Proc Natl Acad Sci U S A

Ca2+-sensor ALG-2 engages ESCRTs to enhance lysosomal membrane resilience to osmotic stress

Authors - Wei Chen
humanWB

Dev Cell

NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.

Authors - Oliver B Davis
human

MBio

Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.

Authors - Ekansh Mittal
mouseWB,RIP,IF

J Neurochem

Reduced circ_lrrc49 in trigeminal ganglion contributes to neuropathic pain in mice by downregulating Ist1 and impairing autophagy

Authors - Qingfeng Tang
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