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OGDHL Recombinant antibody

OGDHL Recombinant Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230228B2

Cat No : 82981-1-RR

Print datasheet

Synonyms

KIAA1290, OGDC E1 like, OGDHL

验证数据展示

  • IF Staining of U2OS using 82981-1-RR

    IF Staining of U2OS using 82981-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using OGDHL antibody (82981-1-RR, Clone: 230228B2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IHC staining of human liver using 82981-1-RR

    IHC staining of human liver using 82981-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 82981-1-RR

    IHC staining of human liver using 82981-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82981-1-RR targets OGDHL in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity Human
Immunogen OGDHL fusion protein Ag10794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name oxoglutarate dehydrogenase-like
Synonyms KIAA1290, OGDC E1 like, OGDHL
Calculated Molecular Weight 1010 aa, 115 kDa
GenBank Accession NumberBC026320
Gene Symbol OGDHL
Gene ID (NCBI) 55753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9ULD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.

实验方案

Product Specific Protocols
IHC protocol for OGDHL antibody 82981-1-RRDownload protocol
IF protocol for OGDHL antibody 82981-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols