OGDHL Recombinant antibody

OGDHL Uni-rAbTM Recombinant Antibody for IHC, FC (Intra), ELISA
Cat No. 82981-1-RR

产品说明书

CloneNo. 230228B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra), ELISA

KIAA1290, EC:1.2.4.2, Alpha-ketoglutarate dehydrogenase-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1-like

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82981-1-RR targets OGDHL in IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 OGDHL fusion protein Ag10794 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 oxoglutarate dehydrogenase-like
别名 KIAA1290, EC:1.2.4.2, Alpha-ketoglutarate dehydrogenase-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1-like
计算分子量 1010 aa, 115 kDa
GenBank蛋白编号BC026320
基因名称 OGDHL
Gene ID (NCBI) 55753
RRIDAB_3670730
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULD0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.

实验方案

Product Specific Protocols
IHC protocol for OGDHL antibody 82981-1-RRDownload protocol
FC protocol for OGDHL antibody 82981-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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