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验证数据展示

  • IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

    IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

    IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

  • FC experiment of Caco-2 using 82981-1-RR (same clone as 82981-1-PBS)

    FC experiment of Caco-2 using 82981-1-RR (same clone as 82981-1-PBS)

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug OGDHL Recombinant antibody (82981-1-RR, Clone:230228B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82981-1-RR

    Affinity and Kinetic Characterization of 82981-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82981-1-RR against Human OGDHL were performed. The affinity constant is 4.98 nM.

    {{ptg:ProductFeaturesInfo}}

OGDHL Recombinant monoclonal antibody, PBS Only

OGDHL Uni-rAb® Recombinant Antibody for IHC, FC (Intra), Indirect ELISA
Cat No. 82981-1-PBS

产品说明书

CloneNo. 230228B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra), Indirect ELISA

别名

230228B2, 2-oxoglutarate dehydrogenase complex component E1-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, Alpha-ketoglutarate dehydrogenase-like, EC:1.2.4.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82981-1-PBS targets OGDHL in IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10794

Product name: Recombinant human OGDHL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 661-1010 aa of BC026320

Sequence: SGQDVERGTFSHRHHVLHDQEVDRRTCVPMNHLWPDQAPYTVCNSSLSEYGVLGFELGYAMASPNALVLWEAQFGDFHNTAQCIIDQFISTGQAKWVRHNGIVLLLPHGMEGMGPEHSSARPERFLQMSNDDSDAYPAFTKDFEVSQLYDCNWIVVNCSTPANYFHVLRRQILLPFRKPLIIFTPKSLLRHPEAKSSFDQMVSGTSFQRVIPEDGAAARAPEQVQRLIFCTGKVYYDLVKERSSQDLEEKVAITRLEQISPFPFDLIKQEAEKYPGAELAWCQEEHKNMGYYDYISPRFMTILRRARPIWYVGRDPAAAPATGNRNTHLVSLKKFLDTAFNLQAFEGKTF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 oxoglutarate dehydrogenase-like
别名 230228B2, 2-oxoglutarate dehydrogenase complex component E1-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, Alpha-ketoglutarate dehydrogenase-like, EC:1.2.4.2
计算分子量 1010 aa, 115 kDa
GenBank蛋白编号BC026320
基因名称 OGDHL
Gene ID (NCBI) 55753
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULD0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.