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验证数据展示

  • IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

    IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

    IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

  • FC experiment of Caco-2 using 82981-1-RR (same clone as 82981-1-PBS)

    FC experiment of Caco-2 using 82981-1-RR (same clone as 82981-1-PBS)

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug OGDHL Recombinant antibody (82981-1-RR, Clone:230228B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82981-1-RR

    Affinity and Kinetic Characterization of 82981-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82981-1-RR against Human OGDHL were performed. The affinity constant is 4.98 nM.

OGDHL Recombinant antibody, PBS Only

OGDHL Uni-rAbTM Recombinant Antibody for IHC, FC (Intra), Indirect ELISA
Cat No. 82981-1-PBS

产品说明书

CloneNo. 230228B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra), Indirect ELISA

别名

KIAA1290, EC:1.2.4.2, Alpha-ketoglutarate dehydrogenase-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1-like

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82981-1-PBS targets OGDHL in IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 OGDHL fusion protein Ag10794 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 oxoglutarate dehydrogenase-like
别名 KIAA1290, EC:1.2.4.2, Alpha-ketoglutarate dehydrogenase-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1-like
计算分子量 1010 aa, 115 kDa
GenBank蛋白编号BC026320
基因名称 OGDHL
Gene ID (NCBI) 55753
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULD0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.