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验证数据展示

  • IF Staining of HeLa using CL488-66698

    IF Staining of HeLa using CL488-66698

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 OCIAD1 antibody (CL488-66698, Clone: 1C10C3 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL488-66698

    IF Staining of human liver cancer using CL488-66698

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 OCIAD1 antibody (CL488-66698, Clone: 1C10C3 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL488-66698

    IF Staining of human liver cancer using CL488-66698

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 OCIAD1 antibody (CL488-66698, Clone: 1C10C3 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated OCIAD1 Monoclonal antibody

OCIAD1 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-66698

产品说明书

CloneNo. 1C10C3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P

别名

Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66698-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66698 targets OCIAD1 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 OCIAD1 fusion protein Ag9977 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 OCIA domain containing 1
别名 Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ
计算分子量 28 kDa
观测分子量 35 kDa
GenBank蛋白编号BC003409
基因名称 OCIAD1
Gene ID (NCBI) 54940
RRIDAB_2934487
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NX40
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 OCIAD1 antibody CL488-66698Download protocol
Standard Protocols
Click here to view our Standard Protocols