验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IHC detected in | mouse liver tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82883-1-RR targets OAS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | OAS1 fusion protein Ag6793 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | 2',5'-oligoadenylate synthetase 1, 40/46kDa |
| 别名 | EC:2.7.7.84, E18/E16, 2H4, 2'-5'-oligoadenylate synthase 1, 2-5A synthase 1 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC000562 |
| 基因名称 | OAS1 |
| Gene ID (NCBI) | 4938 |
| RRID | AB_3670601 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P00973 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OAS1 antibody 82883-1-RR | Download protocol |
| IHC protocol for OAS1 antibody 82883-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
