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验证数据展示

  • WB analysis using 82883-1-RR (same clone as 82883-1-PBS)

    WB analysis using 82883-1-RR (same clone as 82883-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82883-1-RR (OAS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 82883-1-RR (same clone as 82883-1-PBS)

    IHC staining of mouse liver using 82883-1-RR (same clone as 82883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82883-1-RR (same clone as 82883-1-PBS)

    FC experiment of HeLa using 82883-1-RR (same clone as 82883-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OAS1 Recombinant antibody (82883-1-RR, Clone:2H4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82883-1-RR

    Affinity and Kinetic Characterization of 82883-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82883-1-RR against Human OAS1 were performed. The affinity constant is below 1 pM.

    {{ptg:ProductFeaturesInfo}}

OAS1 Recombinant monoclonal antibody, PBS Only

OAS1 Uni-rAb® Recombinant Antibody for Indirect ELISA, IHC, WB
Cat No. 82883-1-PBS

产品说明书

CloneNo. 2H4

宿主/亚型

Rabbit / IgG

种属反应性

mouse, human

应用

Indirect ELISA, IHC, WB

别名

EC:2.7.7.84, E18/E16, 2H4, 2'-5'-oligoadenylate synthase 1, 2-5A synthase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82883-1-PBS targets OAS1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, human samples.

经测试应用 Indirect ELISA, IHC, WB Application Description
经测试反应性 mouse, human
免疫原

CatNo: Ag6793

Product name: Recombinant human OAS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC000562

Sequence: MMDLRNTPAKSLDKFIEDYLLPDTCFRMQINHAIDIICGFLKERCFRGSSYPVCVSKVVKGGSSGKGTTLRGRSDADLVVFLSPLTTFQDQLNRRGEFIQEIRRQLEACQRERAFSVKFEVQAPRWGNPRALSFVLSSLQLGEGVEFDVLPAFDALGQLTGSYKPNPQIYVKLIEECTDLQKEGEFSTCFTELQRDFLKQRPTKLKSLIRLVKHWYQNCKKKLGKLPPQYALELLTVYAWERGSMKTHFNTAQGFRTVLELVINYQQLCIYWTKYYDFKNPIIEKYLRRQLTKPRPVILDPADPTGNLGGGDPKGWRQLAQEAEAWLNYPCFKNWDGSPVSSWILLVRPPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 2',5'-oligoadenylate synthetase 1, 40/46kDa
别名 EC:2.7.7.84, E18/E16, 2H4, 2'-5'-oligoadenylate synthase 1, 2-5A synthase 1
计算分子量 46 kDa
观测分子量38 kDa
GenBank蛋白编号BC000562
基因名称 OAS1
Gene ID (NCBI) 4938
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00973
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009).