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验证数据展示

  • WB analysis using 67132-1-Ig (same clone as 67132-1-PBS)

    WB analysis using 67132-1-Ig (same clone as 67132-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67132-1-Ig (Ninein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 67132-1-Ig (same clone as 67132-1-PBS)

    IF Staining of MDCK using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 67132-1-Ig (Ninein antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Ninein Monoclonal antibody, PBS Only

Ninein Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67132-1-PBS

产品说明书

CloneNo. 1B10A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, canine

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

NIN, 1B10A8, Glycogen synthase kinase 3 beta-interacting protein, GSK3B interacting protein, GSK3B-interacting protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67132-1-PBS targets Ninein in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, canine samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, canine
免疫原 Ninein fusion protein Ag14652 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ninein (GSK3B interacting protein)
别名 NIN, 1B10A8, Glycogen synthase kinase 3 beta-interacting protein, GSK3B interacting protein, GSK3B-interacting protein
计算分子量 2090 aa, 243 kDa
观测分子量 220 kDa
GenBank蛋白编号BC034708
基因名称 Ninein
Gene ID (NCBI) 51199
RRIDAB_2882431
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N4C6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.