验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, NCCIT cells, HT-29 cells |
| Positive IHC detected in | mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67132-1-Ig targets Ninein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, canine |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag14652 Product name: Recombinant human Ninein protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1870-2020 aa of BC034708 Sequence: VRQLESNLLPKHQKHLNPSGTMNPTEQEKLSLKRECDQFQKEQSPANRKVSQMNSLEQELETIHLENEGLKKKQVKLDEQLMEMQHLRSTATPSPSPHAWDLQLLQQQACPMVPREQFLQLQRQLLQAERINQHLQEELENRTSETNTPQ 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ninein (GSK3B interacting protein) |
| 别名 | NIN, 1B10A8, Glycogen synthase kinase 3 beta-interacting protein, GSK3B interacting protein, GSK3B-interacting protein |
| 计算分子量 | 2090 aa, 243 kDa |
| 观测分子量 | 220 kDa |
| GenBank蛋白编号 | BC034708 |
| 基因名称 | Ninein |
| Gene ID (NCBI) | 51199 |
| RRID | AB_2882431 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8N4C6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Ninein antibody 67132-1-Ig | Download protocol |
| IHC protocol for Ninein antibody 67132-1-Ig | Download protocol |
| WB protocol for Ninein antibody 67132-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method
| ||
Elife Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development |
