×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 27737-1-AP (same clone as 27737-1-PBS)

    WB analysis using 27737-1-AP (same clone as 27737-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27737-1-AP (Nexilin antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

    IHC staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

    IHC staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

    IF Staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

    IF Staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

Nexilin Polyclonal antibody, PBS Only

Nexilin Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 27737-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

27737-1-PBS targets Nexilin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26938

Product name: Recombinant human NEXN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 109-361 aa of BC114444

Sequence: MEKQRQEEQRKRTEEERKRRIEQDMLEKRKIQRELAKRAEQIEDINNTGTESASEEGDDSLLITVVPVKSYKTSGKMKKNFEDLEKEREEKERIKYEEDKRIRYEEQRPSLKEAKCLSLVMDDEIESEAKKESLSPGKLKLTFEELERQRQENRKKQAEEEARKRLEEEKRAFEEARRQMVNEDEENQDTAKIFKGYRPGKLKLSFEEMERQRREDEKRKAEEEARRRIEEEKKAFAEARRNMVVDDDSPEMY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nexilin (F actin binding protein)
别名
计算分子量 675 aa, 81 kDa
观测分子量 80-85 kDa
GenBank蛋白编号BC114444
基因名称 Nexilin
Gene ID (NCBI) 91624
RRIDAB_3085991
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ0ZGT2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nexilin, Also known as NEXN, NELIN, is a filamentous actin-binding protein that localizes to focal contacts and may be involved in cell adhesion and migration(PMID: 15823560). Nexilin is abundantly expressed in heart and skeletal muscle and has an essential role in the maintenance of Z line and sarcomere integrity(PMID: 12053183, 19881492). Mutations in Nexilin have been associated with cardiomyopathy dilated type 1CC (CMD1CC) and cardiomyopathy familial hypertrophic type 20 (CMH20)(PMID: 19881492, 20970104).

||
New chat

Able™

正在加载，请稍候...