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验证数据展示

  • WB analysis using 27737-1-AP

    WB analysis using 27737-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27737-1-AP (Nexilin antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 27737-1-AP

    IHC staining of mouse heart using 27737-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 27737-1-AP

    IHC staining of mouse heart using 27737-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 27737-1-AP

    IF Staining of mouse heart using 27737-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse heart using 27737-1-AP

    IF Staining of mouse heart using 27737-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Nexilin Polyclonal antibody

Nexilin Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 27737-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

F actin binding protein, NELIN, Nexilin, NEXN

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27737-1-AP targets Nexilin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag26938

Product name: Recombinant human NEXN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 109-361 aa of BC114444

Sequence: MEKQRQEEQRKRTEEERKRRIEQDMLEKRKIQRELAKRAEQIEDINNTGTESASEEGDDSLLITVVPVKSYKTSGKMKKNFEDLEKEREEKERIKYEEDKRIRYEEQRPSLKEAKCLSLVMDDEIESEAKKESLSPGKLKLTFEELERQRQENRKKQAEEEARKRLEEEKRAFEEARRQMVNEDEENQDTAKIFKGYRPGKLKLSFEEMERQRREDEKRKAEEEARRRIEEEKKAFAEARRNMVVDDDSPEMY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nexilin (F actin binding protein)
别名 F actin binding protein, NELIN, Nexilin, NEXN
计算分子量 675 aa, 81 kDa
观测分子量 80-85 kDa
GenBank蛋白编号BC114444
基因名称 Nexilin
Gene ID (NCBI) 91624
RRIDAB_3085991
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ0ZGT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nexilin, Also known as NEXN, NELIN, is a filamentous actin-binding protein that localizes to focal contacts and may be involved in cell adhesion and migration(PMID: 15823560). Nexilin is abundantly expressed in heart and skeletal muscle and has an essential role in the maintenance of Z line and sarcomere integrity(PMID: 12053183, 19881492). Mutations in Nexilin have been associated with cardiomyopathy dilated type 1CC (CMD1CC) and cardiomyopathy familial hypertrophic type 20 (CMH20)(PMID: 19881492, 20970104).

实验方案

Product Specific Protocols
IF protocol for Nexilin antibody 27737-1-APDownload protocol
IHC protocol for Nexilin antibody 27737-1-APDownload protocol
WB protocol for Nexilin antibody 27737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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