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验证数据展示

  • WB analysis of U-937 using 85965-5-RR (same clone as 85965-5-PBS)

    WB analysis of U-937 using 85965-5-RR (same clone as 85965-5-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 85965-5-RR (ELA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85965-5-PBS in a different storage buffer formulation.

  • WB analysis of HL-60 using 85965-5-RR (same clone as 85965-5-PBS)

    WB analysis of HL-60 using 85965-5-RR (same clone as 85965-5-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 85965-5-RR (ELA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85965-5-PBS in a different storage buffer formulation.

  • Affinity of 85965-5-RR

    Affinity of 85965-5-RR

    Biolayer interferometry (BLI) kinetic assays of 85965-5-RR against Human Neutrophil Elastase were performed. The affinity constant is 2.51 nM.

Neutrophil Elastase Recombinant monoclonal antibody, PBS Only

Neutrophil Elastase Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85965-5-PBS

产品说明书

CloneNo. 253414H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

ELA2, ELANE, EC:3.4.21.37, Elastase 2, HLE

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ELANE/ELA2 Ready-To-Use IHC Kit. ELANE/ELA2 primary antibody included.

产品信息

85965-5-PBS targets Neutrophil Elastase in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag39624

Product name: Recombinant human ELA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 206-267 aa of NM_001972.4

Sequence: LVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 elastase 2, neutrophil
别名 ELA2, ELANE, EC:3.4.21.37, Elastase 2, HLE
计算分子量29KD
观测分子量30 kDa
GenBank蛋白编号NM_001972.4
基因名称 ELA2
Gene ID (NCBI) 1991
RRIDAB_3744512
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08246
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.

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