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NeutraControl IFN gamma Monoclonal antibody

NeutraControl IFN gamma Monoclonal Antibody for Non-Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Non-Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

2F11G6

Cat No : 69507-1-Ig

Print datasheet

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

验证数据展示

  • Non-Neutralization experiment of HT-29 cell using 69507-1-Ig

    Non-Neutralization experiment of HT-29 cell using 69507-1-Ig

    Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL.The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

  • ELISA experiment of Recombinant protein using 69507-1-Ig

    ELISA experiment of Recombinant protein using 69507-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

产品信息

69507-1-Ig targets IFN gamma in ELISA, Non-Neutralization applications and shows reactivity with Human samples.

Tested Applications Non-Neutralization, ELISA
Tested Reactivity Human
Immunogen human Humankine IFN gamma protein HZ-1301 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IFN gamma
Synonyms IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Gene Symbol IFNG
Gene ID (NCBI) 3458
ENSEMBL Gene IDENSG00000111537
RRIDAB_2918850
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody is a neutralizing control antibody for IFN gamma, the immunogen is the same as Neutrakine 69007-1-Ig but could not neutralize human IFN gamma.