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验证数据展示

  • Non-Neutralization experiment of HT-29 cell using 69507-1-Ig (same clone as 69507-1-PBS)

    Non-Neutralization experiment of HT-29 cell using 69507-1-Ig (same clone as 69507-1-PBS)

    Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69507-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69507-1-Ig (same clone as 69507-1-PBS)

    ELISA experiment of Recombinant protein using 69507-1-Ig (same clone as 69507-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69507-1-PBS in a different storage buffer formulation.

NeutraControl IFN-gamma Monoclonal antibody, PBS Only

NeutraControl IFN-gamma Monoclonal Antibody for ELISA, Non-Neutralization
Cat No. 69507-1-PBS

产品说明书

CloneNo. 2F11G6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

ELISA, Non-Neutralization

别名

NeutraControl IFN gamma, IFNG, 2F11G6, IFG, IFN gamma

缓冲液配方:  PBS Only
Sterile PBS
PBS Only
偶联物:  Unconjugated
规格价格库存

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69507-1-Ig

产品信息

69507-1-PBS targets IFN-gamma in applications and shows reactivity with human samples.

经测试应用 ELISA, Non-Neutralization Application Description
经测试反应性 human
免疫原 human Humankine IFN-gamma protein HZ-1301 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 IFN gamma
别名 NeutraControl IFN gamma, IFNG, 2F11G6, IFG, IFN gamma
基因名称 IFNG
Gene ID (NCBI) 3458
ENSEMBL Gene IDENSG00000111537
偶联类型 Unconjugated
纯化方式Protein G purification
储存缓冲液 PBS Only, pH 7.4.
储存Store at -80°C.

背景介绍

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody is a neutralizing control antibody for IFN gamma, the immunogen is the same as Neutrakine 69007-1-Ig but could not neutralize human IFN gamma.