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CoraLite®555-conjugated NeutraKine® IFN Gamma Monoclonal antibody

NeutraKine® IFN Gamma Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1E10G7

Cat No : CL555-69007

Print datasheet

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

验证数据展示

  • FC experiment of Jurkat using CL555-69007

    FC experiment of Jurkat using CL555-69007

    1X10^6 PMA and ionomycin treated Jurkat cells were intracellularly stained with 0.25 ug CoraLite®555 Anti-Human NeutraKine® IFN Gamma (CL555-69007, Clone:1E10G7), and 0.25 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inPMA and ionomycin treated Jurkat cells
For other applications, we recommend the unconjugated version of this antibody, 69007-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-69007 targets NeutraKine® IFN Gamma in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen NeutraKine® IFN Gamma fusion protein HZ-1301 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IFN gamma
Synonyms IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Gene Symbol IFNG
Gene ID (NCBI) 3458
ENSEMBL Gene IDENSG00000111537
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody can be used to neutralize the bioactivity of Interferon gamma.

实验方案

Product Specific Protocols
FC protocol for CL555 NeutraKine® IFN Gamma antibody CL555-69007Download protocol
Standard Protocols
Click here to view our Standard Protocols