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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using CL647-69007

    FC experiment of human PBMCs using CL647-69007

    1X10^6 PMA+ionomycin+protein transport inhibitors treated for 6h human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Human IFN Gamma (CL647-69007, Clone:1E10G7), and 0.25 ug CoraLite®488 Anti-Human CD3 (UCHT1) (CL488-65151, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated IFN Gamma Monoclonal antibody

IFN Gamma Monoclonal Antibody for FC (Intra)
Cat No. CL647-69007

产品说明书

CloneNo. 1E10G7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

IFG, IFI, IFN gamma, IFN γ, IFNG, IFN-gamma, IFNγ, IFN-γ, Immune interferon, Interferon gamma, interferon, gamma, NeutraKine® IFN Gamma

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inPMA+ionomycin+protein transport inhibitors for 6h human PBMCs
Positive FC detected inPMA+ionomycin+protein transport inhibitors for 6h human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 69007-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-69007 targets IFN Gamma in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 fusion protein 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 IFN gamma
别名 IFG, IFI, IFN gamma, IFN γ, IFNG, IFN-gamma, IFNγ, IFN-γ, Immune interferon, Interferon gamma, interferon, gamma, NeutraKine® IFN Gamma
基因名称 IFNG
Gene ID (NCBI) 3458
ENSEMBL Gene IDENSG00000111537
RRIDAB_2920321
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP01579
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IFN Gamma antibody CL647-69007Download protocol
Standard Protocols
Click here to view our Standard Protocols