CoraLite® Plus 488-conjugated NeuN Monoclonal antibody
NeuN Monoclonal Antibody for FC (Intra), IF/ICC,IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
FC (Intra), IF/ICC,IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3A4C1
验证数据展示
经过测试的应用
Positive IF detected in | mouse cerebellum tissue, mouse brain tissue |
Positive FC detected in | U-87 MG cells, SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
CL488-66836 targets NeuN in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | FC (Intra), IF/ICC,IF-P |
Cited Applications | IF |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | rat |
Immunogen | NeuN fusion protein Ag28016 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | hexaribonucleotide binding protein 3 |
Synonyms | cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3 |
GenBank Accession Number | NM_001082575 |
Gene Symbol | hCG_1776007 |
Gene ID (NCBI) | 146713 |
RRID | AB_2883386 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 NeuN antibody CL488-66836 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Exp Eye Res Geranylgeranylacetone-induced heat shock protein70 expression reduces retinal ischemia-reperfusion injury through PI3K/AKT/mTOR signaling |