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验证数据展示

  • IF Staining of rat brain using 84401-4-RR

    IF Staining of rat brain using 84401-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 84401-4-RR

    WB analysis using 84401-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84401-4-RR

    WB analysis using 84401-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84401-4-RR

    WB analysis using 84401-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 84401-4-RR

    IHC staining of mouse cerebellum using 84401-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NeuN antibody included
  • IHC staining of mouse cerebellum using 84401-4-RR

    IHC staining of mouse cerebellum using 84401-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NeuN antibody included
  • IHC staining of rat cerebellum using 84401-4-RR

    IHC staining of rat cerebellum using 84401-4-RR

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NeuN antibody included
  • IHC staining of rat cerebellum using 84401-4-RR

    IHC staining of rat cerebellum using 84401-4-RR

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NeuN antibody included
  • IF Staining of rat brain using 84401-4-RR

    IF Staining of rat brain using 84401-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 84401-4-RR

    IF Staining of mouse brain using 84401-4-RR

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84401-4-RR

    Affinity and Kinetic Characterization of 84401-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84401-4-RR against Human NeuN were performed. The affinity constant is 0.986 nM.

NeuN Recombinant monoclonal antibody

NeuN Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 84401-4-RR

产品说明书

CloneNo. 241461G7
引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, IF-Fro, ELISA and More (1)

别名

241461G7, Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, pig brain tissue, rat cerebellum tissue, rat brain tissue
Positive IHC detected inrat cerebellum tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF-Fro detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84401-4-RR targets NeuN in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, IF-Fro, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性rat
免疫原

CatNo: Ag25689

Product name: Recombinant human NeuN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of NM_001082575

Sequence: MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTPAQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hexaribonucleotide binding protein 3
别名 241461G7, Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen
观测分子量 46-52 kDa
GenBank蛋白编号NM_001082575
基因名称 NeuN
Gene ID (NCBI) 146713
RRIDAB_3671930
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDA6NFN3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

实验方案

Product Specific Protocols
IF protocol for NeuN antibody 84401-4-RRDownload protocol
IHC protocol for NeuN antibody 84401-4-RRDownload protocol
WB protocol for NeuN antibody 84401-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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