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验证数据展示

  • IF Staining of rat brain using 84401-4-RR (same clone as 84401-4-PBS)

    IF Staining of rat brain using 84401-4-RR (same clone as 84401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • WB analysis using 84401-4-RR (same clone as 84401-4-PBS)

    WB analysis using 84401-4-RR (same clone as 84401-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • WB analysis using 84401-4-RR (same clone as 84401-4-PBS)

    WB analysis using 84401-4-RR (same clone as 84401-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

    IHC staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NeuN antibody included
  • IHC staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

    IHC staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NeuN antibody included
  • IF Staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

    IF Staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 84401-4-RR (same clone as 84401-4-PBS)

    IF Staining of rat brain using 84401-4-RR (same clone as 84401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84401-4-RR (same clone as 84401-4-PBS)

    IF Staining of mouse brain using 84401-4-RR (same clone as 84401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84401-4-RR

    Affinity and Kinetic Characterization of 84401-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84401-4-RR against Human NeuN were performed. The affinity constant is 0.986 nM.

NeuN Recombinant monoclonal antibody, PBS Only

NeuN Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA
Cat No. 84401-4-PBS

产品说明书

CloneNo. 241461G7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, IF-Fro, Indirect ELISA

别名

241461G7, Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.

产品信息

84401-4-PBS targets NeuN in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, IF-Fro, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag25689

Product name: Recombinant human NeuN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of NM_001082575

Sequence: MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTPAQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hexaribonucleotide binding protein 3
别名 241461G7, Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen
观测分子量 46-52 kDa
GenBank蛋白编号NM_001082575
基因名称 NeuN
Gene ID (NCBI) 146713
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDA6NFN3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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