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验证数据展示

  • WB analysis using 80210-13-RR

    WB analysis using 80210-13-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of NCCIT using 80210-13-RR

    IF Staining of NCCIT using 80210-13-RR

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Nanog antibody (80210-13-RR, Clone: 250067F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80210-13-RR

    Affinity and Kinetic Characterization of 80210-13-RR

    Biolayer interferometry (BLl) kinetic assays of 80210-13-RR against Human Nanog were performed. The affinity constant is 11.0 nM.

Nanog Recombinant antibody

Nanog Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80210-13-RR

产品说明书

CloneNo. 250067F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

hNanog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse brain tissue, rat brain tissue, C2C12 cells
Positive IF/ICC detected inNCCIT cells

This antibody is not recommended for WB detection of human samples.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80210-13-RR targets Nanog in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Nanog fusion protein Ag5645 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Nanog homeobox
别名 hNanog
计算分子量 35 kDa
观测分子量35-40 kDa
GenBank蛋白编号BC160187
基因名称 Nanog
Gene ID (NCBI) 79923
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H9S0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. There are two kinds of variants that can be recognized by NANOG, one is a normal form (~39 kDa), the other is a post-translation modified form (~48 kDa) (21136380 ). Nanog has two isoforms with molecular weights of 34.4 kDa and 31.9 kDa. (PMID: 21969378)

实验方案

Product Specific Protocols
WB protocol for Nanog antibody 80210-13-RRDownload protocol
IF protocol for Nanog antibody 80210-13-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols