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验证数据展示

  • WB analysis using 68399-1-Ig (same clone as 68399-1-PBS)

    WB analysis using 68399-1-Ig (same clone as 68399-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68399-1-Ig (NVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68399-1-Ig (same clone as 68399-1-PBS)

    FC experiment of HeLa using 68399-1-Ig (same clone as 68399-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug NVL Monoclonal antibody (68399-1-Ig, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68399-1-PBS

    FC experiment of HeLa using 68399-1-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug NVL Monoclonal antibody, PBS Only (68399-1-PBS, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NVL Monoclonal antibody, PBS Only

NVL Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68399-1-PBS

产品说明书

CloneNo. 1D8G4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

1D8G4, Nuclear valosin-containing protein-like, nuclear VCP like, Nuclear VCP like protein, Nuclear VCP-like protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68399-1-PBS targets NVL in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9321

Product name: Recombinant human NVL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 309-659 aa of BC012105

Sequence: LPSKGVQEERLGTEPTSETQDELQRLLGLLRDQDPLSEEQMQGLCIELNDFIVALSSVQPSAKREGFVTVPNVTWADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETGASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALRQEMARQKSGNEKGELKVSHKHFEEAFKKVRSSISKKDQIMYERLQESLSR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear VCP-like
别名 1D8G4, Nuclear valosin-containing protein-like, nuclear VCP like, Nuclear VCP like protein, Nuclear VCP-like protein
计算分子量856aa,95 kDa; 659aa,73 kDa
观测分子量 95 kDa
GenBank蛋白编号BC012105
基因名称 NVL
Gene ID (NCBI) 4931
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15381
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for NVL antibody 68399-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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