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验证数据展示

  • WB analysis using 68399-1-Ig

    WB analysis using 68399-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68399-1-Ig (NVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of HeLa using 68399-1-Ig

    FC experiment of HeLa using 68399-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug NVL Monoclonal antibody (68399-1-Ig, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NVL Monoclonal antibody

NVL Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68399-1-Ig

产品说明书

CloneNo. 1D8G4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

1D8G4, Nuclear valosin-containing protein-like, nuclear VCP like, Nuclear VCP like protein, Nuclear VCP-like protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68399-1-Ig targets NVL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9321

Product name: Recombinant human NVL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 309-659 aa of BC012105

Sequence: LPSKGVQEERLGTEPTSETQDELQRLLGLLRDQDPLSEEQMQGLCIELNDFIVALSSVQPSAKREGFVTVPNVTWADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETGASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALRQEMARQKSGNEKGELKVSHKHFEEAFKKVRSSISKKDQIMYERLQESLSR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear VCP-like
别名 1D8G4, Nuclear valosin-containing protein-like, nuclear VCP like, Nuclear VCP like protein, Nuclear VCP-like protein
计算分子量856aa,95 kDa; 659aa,73 kDa
观测分子量 95 kDa
GenBank蛋白编号BC012105
基因名称 NVL
Gene ID (NCBI) 4931
RRIDAB_3085116
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15381
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NVL(nuclear VCP-like) , also named as NVL2, belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. NVL plays an essential role in ribosome biogenesis by specifically associating with ribosomal protein L5 and modulating the function of DOB1, thereby participating in the assembly of the 60S ribosomal subunit. Additionally, NVL is required for telomerase assembly and the regulation of telomerase activity, and is involved in pre-rRNA processing. This protein has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for NVL antibody 68399-1-IgDownload protocol
WB protocol for NVL antibody 68399-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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