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验证数据展示

  • WB analysis using 15027-1-AP (same clone as 15027-1-PBS)

    WB analysis using 15027-1-AP (same clone as 15027-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15027-1-AP (NUP85 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 15027-1-AP (same clone as 15027-1-PBS)

    IHC staining of mouse testis using 15027-1-AP (same clone as 15027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUP85 antibody included
  • IHC staining of rat brain using 15027-1-AP (same clone as 15027-1-PBS)

    IHC staining of rat brain using 15027-1-AP (same clone as 15027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUP85 antibody included
  • IF Staining of U2OS using 15027-1-AP (same clone as 15027-1-PBS)

    IF Staining of U2OS using 15027-1-AP (same clone as 15027-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NUP85 antibody (15027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

NUP85 Polyclonal antibody, PBS Only

NUP85 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15027-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NUP85 Ready-To-Use IHC Kit. NUP85 primary antibody included.

产品信息

15027-1-PBS targets NUP85 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NUP85 fusion protein Ag7158 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleoporin 85kDa
别名
计算分子量 75 kDa
观测分子量60 kDa
GenBank蛋白编号BC000697
基因名称 NUP85
Gene ID (NCBI) 79902
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BW27
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. NPCs are key for nucleocytoplasmic transport, but also regulate the mitotic machinery, transcription and chromatin organization through transport-independent mechanisms. The NUP107-160 complex is acknowledged to contribute to the assembly and maintenance of the NPC structure. Members of this largest subcomplex of the NPC associate with kinetochores, mitotic spindles, centrosomes and mitotic checkpoint regulators for proper completion of the cell cycle . Downregulation of NUP107-160 subcomplex members resulted in defective cytokinesis, compromised microtubule structures, altered cytoskeletal dynamics, impaired chromosome segregation and differentiation. (PMID:34170319)