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验证数据展示

  • WB analysis using 86622-1-RR (same clone as 86622-1-PBS)

    WB analysis using 86622-1-RR (same clone as 86622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86622-1-RR (NUP153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86622-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02679-1

    Sandwich ELISA sample test of MP02679-1

    The mean NUP153 concentration was determined to be 30.07 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load, 97.51 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load and 33.07 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02679-1

    Sandwich ELISA standard curve of MP02679-1

    Sandwich ELISA standard curve of MP02679-1, Human NUP153 Recombinant Matched Antibody Pair - PBS only. 86622-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5519. 86622-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • IF Staining of HeLa using 86622-1-RR (same clone as 86622-1-PBS)

    IF Staining of HeLa using 86622-1-RR (same clone as 86622-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUP153 antibody (86622-1-RR, Clone: 251519G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86622-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86622-1-RR

    Affinity and Kinetic Characterization of 86622-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86622-1-RR against Human NUP153 were performed. The affinity constant is 2.32 nM.

NUP153 Recombinant monoclonal antibody, PBS Only (Capture)

NUP153 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86622-1-PBS

产品说明书

CloneNo. 251519G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

153 kDa nucleoporin, HNUP153, N153, nucleoporin 153kDa, Nucleoporin Nup153

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86622-1-PBS targets NUP153 as part of a matched antibody pair:

MP02679-1: 86622-1-PBS capture and 86622-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5519

Product name: Recombinant human NUP153 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1125-1475 aa of BC052965

Sequence: EEPKCQPVFSFGNSEQTKDENSSKSTFSFSMTKPSEKESEQPAKATFAFGAQTSTTADQGAAKPVFSFLNNSSSSSSTPATSAGGGIFGSSTSSSNPPVATFVFGQSSNPVSSSAFGNTAESSTSQSLLFSQDSKLATTSSTGTAVTPFVFGPGASSNNTTTSGFGFGATTTSSSAGSSFVFGTGPSAPSASPAFGANQTPTFGQSQGASQPNPPGFGSISSSTALFPTGSQPAPPTFGTVSSSSQPPVFGQQPSQSAFGSGTTPNSSSAFQFGSSTTNFNFTNNSPSGVFTFGANSSTPAASAQPSGSGGFPFNQSPAAFTVGSNGKNVFSSSGTSFSGRKIKTAVRRRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleoporin 153kDa
别名 153 kDa nucleoporin, HNUP153, N153, nucleoporin 153kDa, Nucleoporin Nup153
计算分子量 154 kDa
观测分子量160-170 kDa
GenBank蛋白编号BC052965
基因名称 NUP153
Gene ID (NCBI) 9972
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49790
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NUP153 is an FG-repeat containing NUP, contains a high-affinity site for importin-β, localizes on the nucleoplasmic face of the nuclear pore and is required for assembly of the nuclear basket and import of a subset of nuclear proteins [PMID:18845677]. NUP153 also facilitates nuclear export of mRNA and ribonucleoprotein particles and may have additional functions within the nucleus [PMID:16195343]. The 53BP1-NUP153/importin-β pathway as an important aspect of the DDR network add to an emerging evidence of subcellular trafficking as an integral part of genome surveillance [PMID:22075984].

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