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验证数据展示

  • IF Staining of HepG2 using CL488-66335

    IF Staining of HepG2 using CL488-66335

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66335 (NUDT21 antibody) at dilution of 1:100.

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  • KD/KO验证

CoraLite® Plus 488-conjugated NUDT21 Monoclonal antibody

NUDT21 Monoclonal Antibody for IF/ICC
Cat No. CL488-66335

产品说明书

CloneNo. 1C4C8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC

别名

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, DKFZp686H1588, Nudix motif 21, NUDT21

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NUDT21 Ready-To-Use IHC Kit. NUDT21 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66335-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66335 targets NUDT21 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12260

Product name: Recombinant human NUDT21 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-222 aa of BC001403

Sequence: MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 21
别名 CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, DKFZp686H1588, Nudix motif 21, NUDT21
计算分子量 26 kDa
观测分子量 27 kDa
GenBank蛋白编号BC001403
基因名称 NUDT21
Gene ID (NCBI) 11051
RRIDAB_2883312
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDO43809
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NUDT21 antibody CL488-66335Download protocol
Standard Protocols
Click here to view our Standard Protocols