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验证数据展示

  • WB analysis of HepG2 using 10681-1-AP (same clone as 10681-1-PBS)

    WB analysis of HepG2 using 10681-1-AP (same clone as 10681-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10681-1-AP (same clone as 10681-1-PBS)

    IP experiment of HEK-293 using 10681-1-AP (same clone as 10681-1-PBS)

    IP result of anti-NUDC (IP:10681-1-AP, 4ug; Detection:10681-1-AP 1:1000) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 10681-1-AP (same clone as 10681-1-PBS)

    IHC staining of human pancreas cancer using 10681-1-AP (same clone as 10681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10681-1-AP (NUDC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUDC antibody included
  • IHC staining of human pancreas cancer using 10681-1-AP (same clone as 10681-1-PBS)

    IHC staining of human pancreas cancer using 10681-1-AP (same clone as 10681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10681-1-AP (NUDC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUDC antibody included
  • IF Staining of HeLa using 10681-1-AP (same clone as 10681-1-PBS)

    IF Staining of HeLa using 10681-1-AP (same clone as 10681-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDC antibody (10681-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NUDC Polyclonal antibody, PBS Only

NUDC Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10681-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

HNUDC, MNUDC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NUDC Ready-To-Use IHC Kit. NUDC primary antibody included.

产品信息

10681-1-PBS targets NUDC in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0998

Product name: Recombinant human NUDC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-331 aa of BC006147

Sequence: MGGEQEEERFDGMLLAMAQQHEGGVQELVNTFFSFLRRKTDFFIGGEEGMAEKLITQTFSHHNQLAQKTRREKRARQEAERREKAERAARLAKEAKSETSGPQIKELTDEEAERLQLEIDQKKDAENHEAQLKNGSLDSPGKQDTEEDEEEDEKDKGKLKPNLGNGADLPNYRWTQTLSELDLAVPFCVNFRLKGKDMVVDIQRRHLRVGLKGQPAIIDGELYNEVKVEESSWLIEDGKVVTVHLEKINKMEWWSRLVSSDPEINTKKINPENSKLSDLDSETRSMVEKMMYDQRQKSMGLPTSDEQKKQEILKKFMDQHPEMDFSKAKFN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear distribution gene C homolog (A. nidulans)
别名 HNUDC, MNUDC
计算分子量 38 kDa
观测分子量 42-45 kDa
GenBank蛋白编号BC006147
基因名称 NUDC
Gene ID (NCBI) 10726
RRIDAB_2282933
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y266
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear distribution protein C (NudC) is a highly conserved dynein/dynactin associated protein implicated in mitosis and cytokinesis. NudC is localized on major mitotic structures, and is involved in regulating spindle formation stability of kinetochore microtubule attachments, and chromosomes congression in early mitosis. NudC also is required for multi-protein transport along axons in neurons.

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