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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83917-2-RR (same clone as 83917-2-PBS)

    WB analysis using 83917-2-RR (same clone as 83917-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83917-2-RR (NUCB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83917-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83917-2-RR (same clone as 83917-2-PBS)

    FC experiment of HepG2 using 83917-2-RR (same clone as 83917-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR, Clone:241037D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83917-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83917-2-RR

    Affinity and Kinetic Characterization of 83917-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83917-2-RR against Human NUCB2/nesfatin-1 were performed. The affinity constant is 8.99 nM.

NUCB2/nesfatin-1 Recombinant antibody, PBS Only

NUCB2/nesfatin-1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83917-2-PBS

产品说明书

CloneNo. 241037D8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

NUCB2, 241037D8, DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83917-2-PBS targets NUCB2/nesfatin-1 in WB, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NUCB2/nesfatin-1 fusion protein Ag25152 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleobindin 2
别名 NUCB2, 241037D8, DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA
计算分子量 50 kDa
观测分子量 45-50 kDa
GenBank蛋白编号NM_005013
基因名称 NUCB2
Gene ID (NCBI) 4925
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP80303
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).