验证数据展示
经过测试的应用
| Positive WB detected in | IMR-32 cells, SH-SY5Y cells |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85375-1-RR targets NTN1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | netrin 1 |
| 别名 | NTN1, Epididymis tissue protein Li 131P, netrin 1, Netrin-1, NTN1L |
| 计算分子量 | 68 kDa |
| 观测分子量 | 65-68 kDa |
| GenBank蛋白编号 | NM_004822 |
| 基因名称 | NTN1 |
| Gene ID (NCBI) | 9423 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95631 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NTN1 is also named as NTN1L. Netrins control the guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor through its association with its receptors, which prevents the initiation of apoptosis. NTN1 is involved in colorectal tumorigenesis by regulating apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NTN1-Specific antibody 85375-1-RR | Download protocol |
| WB protocol for NTN1-Specific antibody 85375-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
