验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human brain tissue, HeLa cells, K-562 cells, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IP | See 1 publications below |
产品信息
11154-1-AP targets NTHL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag1631 Product name: Recombinant human NTHL1 protein Source: e coli.-derived, Unknow Tag: N/A Domain: C16022 of BC003014 Sequence: 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nth endonuclease III-like 1 (E. coli) |
| 别名 | NTH1, NTHL1, OCTS3 |
| 计算分子量 | 34 aa, 3 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC003014 |
| 基因名称 | NTHL1 |
| Gene ID (NCBI) | 4913 |
| RRID | AB_2283005 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78549 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NTHL1 antibody 11154-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FASEB J Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats. | ||
Respir Res Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase. | ||
NAR Cancer The DNA repair function of BCL11A suppresses senescence and promotes continued proliferation of triple-negative breast cancer cells | ||
Nucleic Acids Res The function of BCL11B in base excision repair contributes to its dual role as an oncogene and a haplo-insufficient tumor suppressor gene |
