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验证数据展示

  • IF Staining of HeLa using CL488-66063

    IF Staining of HeLa using CL488-66063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488-conjugated NTF2 antibody (CL488-66063, Clone: 4H7F9 ) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated NTF2 Monoclonal antibody

NTF2 Monoclonal Antibody for IF
Cat No. CL488-66063

产品说明书

CloneNo. 4H7F9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF

别名

NTF 2, NTF2, nuclear transport factor 2, NUTF2, Placental protein 15, PP15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66063-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66063 targets NTF2 in IF applications and shows reactivity with human samples.

经测试应用 IF Application Description
经测试反应性 human
免疫原 NTF2 fusion protein Ag7879 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear transport factor 2
别名 NTF 2, NTF2, nuclear transport factor 2, NUTF2, Placental protein 15, PP15
计算分子量 14 kDa
观测分子量 13 kDa
GenBank蛋白编号BC002348
基因名称 NTF2
Gene ID (NCBI) 10204
RRIDAB_2883246
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61970
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NTF2 antibody CL488-66063Download protocol
Standard Protocols
Click here to view our Standard Protocols