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- KD/KO Validated
NTF2 Monoclonal antibody
NTF2 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
4H7F9
验证数据展示
经过测试的应用
| Positive WB detected in | Hela cells, HepG2 cells, human heart tissue |
| Positive IHC detected in | human placenta tissue, human breast cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
The immunogen of 66063-1-Ig is NTF2 Fusion Protein expressed in E. coli.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | NTF2 fusion protein Ag7879 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | nuclear transport factor 2 |
| Synonyms | NTF 2, NTF2, nuclear transport factor 2, NUTF 2, NUTF2, Placental protein 15, PP15 |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC002348 |
| Gene Symbol | NUTF2 |
| Gene ID (NCBI) | 10204 |
| RRID | AB_11042770 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P61970 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NTF2 antibody 66063-1-Ig | Download protocol |
| IHC protocol for NTF2 antibody 66063-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol NTF2 Upregulation in HNSCC: a Predictive Marker and Potential Therapeutic Target Associated With Immune Infiltration.
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