CoraLite® Plus 488-conjugated NT5E,CD73 Monoclonal antibody
NT5E,CD73 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat and More (1)
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G5G8
验证数据展示
经过测试的应用
| Positive IF detected in | human tonsillitis tissue |
For other applications, we recommend the unconjugated version of this antibody, 67789-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
CL488-67789 targets NT5E,CD73 in IF applications and shows reactivity with Human, Rat samples.
| Tested Applications | IF-P |
| Cited Applications | IF |
| Tested Reactivity | Human, Rat |
| Cited Reactivity | human |
| Immunogen | NT5E,CD73 fusion protein Ag30616 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | 5'-nucleotidase, ecto (CD73) |
| Synonyms | 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E,CD73, NTE |
| Calculated Molecular Weight | 29 kDa, 63 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC015940 |
| Gene Symbol | NT5E |
| Gene ID (NCBI) | 4907 |
| RRID | AB_2919547 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P21589 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NT5E,CD73 antibody CL488-67789 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol NT5E upregulation in head and neck squamous cell carcinoma: A novel biomarker on cancer-associated fibroblasts for predicting immunosuppressive tumor microenvironment | ||
Front Pharmacol Pan-cancer analysis identifies NT5E as a novel prognostic biomarker on cancer-associated fibroblasts associated with unique tumor microenvironment |