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验证数据展示

  • WB analysis using 84795-1-RR

    WB analysis using 84795-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84795-1-RR (NSUN7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 84795-1-RR

    IHC staining of mouse testis using 84795-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84795-1-RR (NSUN7 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84795-1-RR

    Affinity and Kinetic Characterization of 84795-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84795-1-RR against Human NSUN7 were performed. The affinity constant is 3.58 nM.

NSUN7 Recombinant antibody

NSUN7 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84795-1-RR

产品说明书

CloneNo. 242358B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

242358B7, FLJ14001

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, U-251 cells, U-87 MG cells, HepG2 cells, human testis tissue, rat liver tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84795-1-RR targets NSUN7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NSUN7 fusion protein Ag11650 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 7
别名 242358B7, FLJ14001
计算分子量 718 aa, 81 kDa
观测分子量81~90 kDa
GenBank蛋白编号BC036568
基因名称 NSUN7
Gene ID (NCBI) 79730
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NE18
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).

实验方案

Product Specific Protocols
WB protocol for NSUN7 antibody 84795-1-RRDownload protocol
IHC protocol for NSUN7 antibody 84795-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols