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验证数据展示

  • IF Staining of HepG2 using 84275-3-RR

    IF Staining of HepG2 using 84275-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 84275-3-RR

    IF Staining of HepG2 using 84275-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

NSUN4 Recombinant monoclonal antibody

NSUN4 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84275-3-RR

产品说明书

CloneNo. 241115G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115G11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84275-3-RR targets NSUN4 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31828

Product name: Recombinant human NSUN4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 26-284 aa of BC014441

Sequence: RYKKKWAATEPKFPAVRLALQNFDMAYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 4
别名 RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115G11
计算分子量 384 aa, 43 kDa
GenBank蛋白编号BC014441
基因名称 NSUN4
Gene ID (NCBI) 387338
RRIDAB_3671821
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96CB9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.

实验方案

Product Specific Protocols
IF protocol for NSUN4 antibody 84275-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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