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验证数据展示

  • IF Staining of HepG2 using 84275-3-RR (same clone as 84275-3-PBS)

    IF Staining of HepG2 using 84275-3-RR (same clone as 84275-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84275-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84275-3-RR (same clone as 84275-3-PBS)

    IF Staining of HepG2 using 84275-3-RR (same clone as 84275-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84275-3-PBS in a different storage buffer formulation.

NSUN4 Recombinant monoclonal antibody, PBS Only

NSUN4 Uni-rAb® Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 84275-3-PBS

产品说明书

CloneNo. 241115G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Indirect ELISA

别名

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115G11

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84275-3-PBS targets NSUN4 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31828

Product name: Recombinant human NSUN4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 26-284 aa of BC014441

Sequence: RYKKKWAATEPKFPAVRLALQNFDMAYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 4
别名 RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115G11
计算分子量 384 aa, 43 kDa
GenBank蛋白编号BC014441
基因名称 NSUN4
Gene ID (NCBI) 387338
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96CB9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.

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