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验证数据展示

  • WB analysis using 29786-1-AP (same clone as 29786-1-PBS)

    WB analysis using 29786-1-AP (same clone as 29786-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29786-1-AP (NSUN4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29786-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 29786-1-AP (same clone as 29786-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 29786-1-AP (same clone as 29786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29786-1-AP (NSUN4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29786-1-PBS in a different storage buffer formulation.

NSUN4 Polyclonal antibody, PBS Only

NSUN4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29786-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29786-1-PBS targets NSUN4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31821

Product name: Recombinant human NSUN4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-284 aa of BC014441

Sequence: RYKKKWAATEPKFPAVRLALQNFDMAYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 4
别名 RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4
计算分子量 384 aa, 43 kDa
观测分子量40 kDa
GenBank蛋白编号BC014441
基因名称 NSUN4
Gene ID (NCBI) 387338
RRIDAB_2935480
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CB9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly. The calculated MW of NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.(PMID:22949673, 24516400)

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