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验证数据展示

  • WB analysis using 29786-1-AP

    WB analysis using 29786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29786-1-AP (NSUN4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 29786-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 29786-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29786-1-AP (NSUN4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NSUN4 Polyclonal antibody

NSUN4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29786-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, SH-SY5Y cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29786-1-AP targets NSUN4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 NSUN4 fusion protein Ag31821 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 4
别名 RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4
计算分子量 384 aa, 43 kDa
观测分子量40 kDa
GenBank蛋白编号BC014441
基因名称 NSUN4
Gene ID (NCBI) 387338
RRIDAB_2935480
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CB9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly. The calculated MW of NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.(PMID:22949673, 24516400)

实验方案

Product Specific Protocols
WB protocol for NSUN4 antibody 29786-1-APDownload protocol
IHC protocol for NSUN4 antibody 29786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols