验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, SH-SY5Y cells, PC-12 cells, HEK-293 cells, Rabbit brain tissue, Rat brain tissue, Mouse brain tissue, Chicken brain tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68061-1-Ig targets NSF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit, chicken |
| 免疫原 |
CatNo: Ag15422 Product name: Recombinant human NSF protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 394-744 aa of BC030613 Sequence: EIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | N-ethylmaleimide-sensitive factor |
| 别名 | 2B6H6, EC:3.6.4.6, NEM-sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, SKD2 |
| 计算分子量 | 744 aa, 83 kDa |
| 观测分子量 | 70-83 kDa |
| GenBank蛋白编号 | BC030613 |
| 基因名称 | NSF |
| Gene ID (NCBI) | 4905 |
| RRID | AB_2918802 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P46459 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NSF antibody 68061-1-Ig | Download protocol |
| IHC protocol for NSF antibody 68061-1-Ig | Download protocol |
| WB protocol for NSF antibody 68061-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
