验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-11345 targets NSD3 in FC (Intra) applications and shows reactivity with human, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag1894 Product name: Recombinant human WHSC1L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC012059 Sequence: MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Wolf-Hirschhorn syndrome candidate 1-like 1 |
| 别名 | WHSC1L1, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3 |
| 计算分子量 | 1437 aa, 162 kDa |
| 观测分子量 | 160-170 kDa, 68-72 kDa |
| GenBank蛋白编号 | BC012059 |
| 基因名称 | WHSC1L1 |
| Gene ID (NCBI) | 54904 |
| RRID | AB_2919027 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZ95 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 NSD3 antibody CL488-11345 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
