- Featured Product
- KD/KO Validated
CoraLite®594-conjugated NRF2, NFE2L2 Polyclonal antibody
NRF2, NFE2L2 Polyclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
Positive FC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
CL594-16396 targets NRF2, NFE2L2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra), IF/ICC |
Cited Applications | IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | rat |
Immunogen | NRF2, NFE2L2 fusion protein Ag9489 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nuclear factor (erythroid-derived 2)-like 2 |
Synonyms | HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2, NRF2, NFE2L2 |
Calculated Molecular Weight | 605 aa, 68 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | BC011558 |
Gene Symbol | NFE2L2 |
Gene ID (NCBI) | 4780 |
RRID | AB_2919830 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16236 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL594 NRF2, NFE2L2 antibody CL594-16396 | Download protocol |
FC protocol for CL594 NRF2, NFE2L2 antibody CL594-16396 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Chin Med Maslinic Acid Ameliorates Myocardial Ischemia Reperfusion Injury-Induced Oxidative Stress via Activating Nrf2 and Inhibiting NF-[Formula: see text]B Pathways |