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验证数据展示

  • WB analysis using 83815-2-RR (same clone as 83815-2-PBS)

    WB analysis using 83815-2-RR (same clone as 83815-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83815-2-RR (NRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83815-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 83815-2-RR (same clone as 83815-2-PBS)

    IHC staining of mouse pancreas using 83815-2-RR (same clone as 83815-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83815-2-RR (NRAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83815-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NRAS antibody included
  • IHC staining of mouse testis using 83815-2-RR (same clone as 83815-2-PBS)

    IHC staining of mouse testis using 83815-2-RR (same clone as 83815-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83815-2-RR (NRAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83815-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NRAS antibody included
  • IHC staining of mouse testis using 83815-2-RR (same clone as 83815-2-PBS)

    IHC staining of mouse testis using 83815-2-RR (same clone as 83815-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83815-2-RR (NRAS antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83815-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NRAS antibody included
  • Affinity and Kinetic Characterization of 83815-2-RR

    Affinity and Kinetic Characterization of 83815-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83815-2-RR against Human NRAS were performed. The affinity constant is 0.152 nM.

NRAS Recombinant monoclonal antibody, PBS Only

NRAS Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 83815-2-PBS

产品说明书

CloneNo. 242810G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

HRAS1, EC:3.6.5.2, GTPase NRas, N ras, NRAS1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NRAS Ready-To-Use IHC Kit. NRAS primary antibody included.

产品信息

83815-2-PBS targets NRAS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1081

Product name: Recombinant human NRAS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-189 aa of BC005219

Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neuroblastoma RAS viral (v-ras) oncogene homolog
别名 HRAS1, EC:3.6.5.2, GTPase NRas, N ras, NRAS1
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC005219
基因名称 NRAS
Gene ID (NCBI) 4893
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01111
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). NRAS is one member of RAS gene family of oncoproteins, which is commonly mutated in melanoma and hematopoietic cancers via mapped on chromosome 1 (PMID: 2327491, PMID: 26990546). NRAS mediates activation of both mitogen-activated protein kinase (MAPK) and PI3K/AKT/MYC signaling (PMID: 17297468). NRAS induced classical MAPK signaling leads to cyclin D1 expression and cell cycle dysregulation and promotion of prosurvival pathways (PMID:7970723,PMID: 18246127). In addition, NRAS effectively prevents Glycogen Synthase Kinase3 (GSK3)-mediated phosphorylation of MYC via PI3K/AKT, which results in enhanced activity of endogenous MYC protein (PMID: 17297468). Mutational NRAS causes Ras-GTP to be in a state of continuous activation, which results in malignant proliferation and metastasis (PMID: 24985059).

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