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验证数据展示

  • WB analysis of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)

    WB analysis of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)

    WB analysis of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)

    WB result of NR3C2 antibody (21854-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR3C2 transfected HEK-293 cells. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)

    IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C2 antibody included
  • IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)

    IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C2 antibody included
  • IP experiment of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)

    IP experiment of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)

    IP result of anti-NR3C2 (IP:21854-1-AP, 4ug; Detection:21854-1-AP 1:500) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21854-1-AP (same clone as 21854-1-PBS)

    IF Staining of HepG2 using 21854-1-AP (same clone as 21854-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NR3C2 Polyclonal antibody, PBS Only

NR3C2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21854-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

MCR, MLR, MR

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NR3C2 Ready-To-Use IHC Kit. NR3C2 primary antibody included.

产品信息

21854-1-PBS targets NR3C2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16410

Product name: Recombinant human NR3C2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC111758

Sequence: METKGYHSLPEGLDMERRWGQVSQAVERSSLGPTERTDENNYMEIVNVSCVSGAIPNNSTQGSSKEKQELLPCLQQDNNRPGILTSDIKTELESKELSATVAESMGLYMDSVRDADYSYEQQNQQGSMSPAKIYQNVEQLVKFYKGNGHRPSTLSCVNTPLRSFMSDSGSSVNGGVMRAIVKSPIMCHEKSPSVCSPLNMTSSVCSPAGINSVSSTTASFGSFPVHSPITQGTPLTCSPNVENRGSRSHSPAHASNVGSPLSSPLSSMKSSISSPPSHCSVKSPVSSPNNVTLRSSVSSPANINNSRCSVSSPSNTNNRSTLSSPAASTVGSICSPVNNAFSYTASGTSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor subfamily 3, group C, member 2
别名 MCR, MLR, MR
计算分子量 984 aa, 107 kDa
观测分子量 94-110 kDa
GenBank蛋白编号BC111758
基因名称 NR3C2
Gene ID (NCBI) 4306
RRIDAB_10858785
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08235
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

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